Genetic Relationships of Mulberry (Morus l.) Using Internal Transcribed Spacer (ITS) Markers
Keywords:
Genetic relationship, Molecular marker, ITS marker, Mulberry, nrDNAAbstract
In the current study, thirty-nine accessions, which belong to 9 species of Morus and 2 species as outgroup (Ficus adhatodifolia, Broussonetia papyrifera) were examined using ITS markers. Genetic variation and phylogenetic relationships among 19 samples from West Sumatra were assessed using the internal transcribed spacer region of nuclear ribosomal DNA (nrDNA ITS). The estimated genetic diversity revealed low genetic variation. Phylogenetic relationships and intraspecific divergence were inferred by neighbour-joining (NJ) analysis was generally resolved. M. alba accessions consistently had one lineage, which indicates the absence of distant geographic isolation and genetic divergence between China accessions and those from other regions. Phylogenetic analysis showed that the monophyletic topology forms a cluster. From the results of this study, it can be concluded that M. alba which is spread in Indonesia comes from the same ancestor. The Reconstruction of phylogenetic tree using the Neighbor-Joining (NJ) method 1000x showed that the ITS region was successfully to predict phylogenetic relationships genus Morus.
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Awashi AK, Nagaraju GM, Naik GV, Kanginakurdu S, Thangavelu K, Javaregowda N .(2004). Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays. BMC Genet 5:1 doi:10.11861471-2156-5-1
Bhattacharya, E. (2005). Molecular assessment of genetic diversity in mulberry (Morus spp.) using PCR based techniques. Ranade S.A., Plant Molecular Biology (Genomics), National Botanical Research Institute, Rana Pratap Marg, Lucknow, U.P. State, 226001, India
Burgess, K.S., M. Morgan, L. Deverno, and B.C. Husband. (2005). Asymmetrical introgression between two Morus species (M. alba, M. rubra) that differ in abundance. Molecular Ecology 14: 3471-3483.
Chen,S.L. and Han,J.-P. (2009). Identification of medicinal plants using DNA barcoding technique. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, No. 151 Malianwa North Road, Haidian District, Beijing 100193, P. R. China
Chen, R, Xu L, Yu M, Liu X, Chen L. (2010). Determination of the Origin and Evolution of Morus (Moraceae) by analyzing the internal transcribed spacer (ITS) sequences. IEEE 1-5
Chen, R. (2014). Systematic position and phylogenetic analyzing of Morus (Moraceae) based on ITS sequences. College of Biotechnology, Southwestern University, Tian Sheng Qiao 216Hao, Chongqing 400716, China
Chen, R. F. and Liu, L. (2016). Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.). Anti-Inflammatory and Antinociception of Mulberry Fruits.PLOS ONE | DOI:10.1371/journal.pone.0153080 April 5, 2016
Daniel, C. and Knoess, W. (2008). Identification of medicinal plants. Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, Bonn 53115, Germany
Doyle JJ, Doyle JL. (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11-15.
Ekasari, T. W. D. (2012). Analisis Keanekaragaman Kultivar Pisang Menggunakan Penanda Pcr-Rflp Pada Internal Transcribed Spacer (Its) Dna Ribosom. Jurnal MIPA 35 (1) (2012)
Kafkas S, Ozgen M, Dogan Y, Ozcan B, Ercisxli S, Serce S. (2008). Molecular characterization of mulberry accessions in Turkey by AFLP markers. J Am Soc Hortic Sci. 133(4):593–597
Kress WJ, Liu AZ, Newman M, Li QJ. (2005). The molecular phylogeny of Alpinia (Zingiberaceac); a complex and polyphyletic genus of Gingers. American Journal of Botany 92: 167-178.
Muellner AN, Samuel R, Johnson SA, Cheek M, Pennington TD, Chase MW. (2003). Molecular phylogenetics of Meliaceae (Sapindales) Based on nuclear and Plastid DNA sequences. American Journal of Botany 90:471-481.
Navia ZI, Suwardi AB, Harmawan T, Syamsuardi, Mukhtar E. (2020). The diversity and contribution of indigenous edible fruit plants to the rural community in the Gayo Highlands, Indonesia. J Agric Rural Dev Trop Subtrop 121 (1): 89-98.
Nepal, M.P. (2008). Systematics and reproductive biology of the genus Morus L. (Moraceae). Ph.D. Dissertation. Kansas State University, Manhattan, KS
Nepal, M.P., and C.J. Ferguson. (2012). Phylogenetics of Morus (Moraceae) inferred from ITS and trnL-trnF sequence data. Systematic Botany 37: 442-450..
Nepal, M.P and D.J. Wichern. (2013). Taxonomic Status Of Red Mulberry (Morus Rubra, Moraceae) At Its Northwestern Boundary. Proceedings of the South Dakota Academy of Science, Vol. 92 (2013)
Nikaido, S. (2010). Mulberry (Morus: Moraceae) hybridization in eastern North America: morphological and molecular evidence. Biology & Earth Science, University of Central Missouri, WC Morris Hall, Room 306, Warrensburg, MO 64093, USA
Quanliang S, Weiguo Z. (2001). Preliminary Study on ITS Sequencing and Characteristics of Mulberry. Canyekexue 27 (2): 140-142.
Rozas J, Sanchez-DelBarrio JC, Messegeur X, Rozas R. (2004). DnaSP, DNA polymorphism analysis by the coalescent and other methods. Bioinformatics 19: 1451-1452.
Saitou N, Nei M. (1989). The Neighbor Joining Method: A new method for constructing phylogenetic trees. Molecular Biology and Evolution 4: 406-425.
Suwardi AB, Navia ZI, Harmawan T, Syamsuardi, Mukhtar E. (2020). Wild edible fruits generate substantial income for local people of the Gunung Leuser National Park, Aceh Tamiang Region. Ethnobot Res Appl. 20: 1-13.
Syamsuardi, Chairul, Murni P. (2018). Analysis of genetic impurty of an original cultivar duku (Lansium parasiticum (Osbeck.) K. C Sahni & Bennet), from Jambi, Indonesia Using ITS and MatK Gene. International Journal of Environment. Agricultural and Biotechnology. 3:441-446
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. (2013). MEGA Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725-2729.
Tanruean., K. and Poolprasert., P. (2018). Identification of mulberry based on DNA barcode. Biology, Pibulsongkram Rajabhat University,156 M.5 Plai Chumpol Sub-District, Muang District, Phitsanulok 65000, Thailand.
Weiguo Z, Yile P, Zhifang Z. (2004). Phylogenetic Relationship of Genus Morus by ITS Sequence Data. Canye Kexue 30 (1): 11-14.
Wu, Z.Y., Liu, J., Provan, J., Wang, H. Chen, C.J., Li, D. Z.and Milne, R.I. (2018). Testing Darwin's transoceanic dispersal hypothesis for the inland nettle family (Urticaceae). Germplasm Bank of Wild Species, Kunming Institute of Botany, 132, Lanhei Road, Kunming, Yunnan 650201, China
Wunderlin, R.P. (1997). Moraceae. Oxford University Press. New
Xu, Y., Zeng, C.X., Zhang, Y.Q., Ren, Y. and Ge, X.J. (2010). DNA barcoding the Flora of Qinling Mt. in China. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, The Chinese Academy of Sciences, Xingke Road 723, Guangzhou, Guangdong 510650, China
Zeng, Q., Chen, H., Wang, X. and He, N. (2013). Classification of Morus species using internal transcribed spacer. outhwest University, State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, 216 Tiansheng Road, Beibei District, Chongqing 400715, China
Zeng Q, Chen H, Chao Z, Minjing H, Tian L, Xiwu Q, Zhonghuai X, Ningjia H. (2015). Definition of Eight Mulberry Species in the Genus Morus by Internal Transcribed Spacer Based Phylogeny. Plos One 0135411.
Zhou ZK, Gilbert MG (2003) Moraceae. In: Wu ZY, Raven PH, Hong DY, editors. Flora of China. Beijing, China & Saint Louis, Missouri: Science Press & Missouri Botanical Garden Press. pp. 22–26.
